In the video, Newton-John seemed shocked when she realized what was in the bright pink, gift-wrapped box.
The jacket and skin-tight pants Newton-John wore in the 1978 film sold separately in the November 2 auction for more than double their estimate and raised $405,700 for the Olivia Newton-John Cancer Research and Wellness Centre in Australia.
Spanx founder Sara Blakely bought the pants, which Newton-John said she had to be sewn into, according to the auction house.
Newton-John wore the outfit for one of the film's final songs, "You're the One That I Want," alongside John Travolta.
She seemed thrilled when the buyer asked if she would put it on display in her cancer center.
On the auction website, a note said that Newton-John wanted to see people enjoying what they'd bought.